Appendix A
Glutathione
S-Transferase as a Biological Marker of Aquatic Contamination Return to the Index Page | Visitor
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The following lists are the Minitab output of the data obtained during the studies detailed in the main text.
1. Study 2.
ROW Treatment* ABS 340 ABS 390 Ratio Original
1 1 0.105 0.038 2.76316 0.199
2 1 0.056 0.023 2.43478 0.222
3 1 0.100 0.042 2.38095 0.239
4 1 0.167 0.060 2.78333 0.286
5 1 0.105 0.034 3.08824 0.225
6 1 0.045 0.021 2.14286 0.228
7 1 0.067 0.027 2.48148 0.260
8 1 0.137 0.053 2.58491 0.405
9 1 0.079 0.027 2.92593 0.191
10 1 0.153 0.058 2.63793 0.319
11 2 0.118 0.038 3.10526 0.175
12 2 0.172 0.057 3.01754 0.172
13 2 0.233 0.074 3.14865 0.191
14 2 0.136 0.046 2.95652 0.255
15 2 0.131 0.045 2.91111 0.250
16 2 0.080 0.029 2.75862 0.239
17 2 0.085 0.028 3.03571 0.218
18 2 0.257 0.089 2.88764 0.313
19 2 0.116 0.037 3.13513 0.273
20 2 0.175 0.057 3.07018 0.261
21 3 0.167 0.065 2.56923 0.233
22 3 0.270 0.095 2.84211 0.255
23 3 0.294 0.101 2.91089 0.275
24 3 0.093 0.037 2.51351 0.227
25 3 0.176 0.060 2.93333 0.249
26 3 0.188 0.067 2.80597 0.404
27 3 0.067 0.015 4.46667 0.226
28 3 0.320 0.097 3.29897 0.581
29 3 0.239 0.075 3.18667 0.432
30 3 0.176 0.058 3.03448 0.329
31 4 0.145 0.053 2.73585 0.089
32 4 0.095 0.034 2.79412 0.054
33 4 0.261 0.088 2.96591 0.087
34 4 0.085 0.035 2.42857 0.067
35 4 0.171 0.064 2.67187 0.113
36 4 0.063 0.019 3.31579 0.142
37 4 0.091 0.028 3.25000 0.225
38 4 0.044 0.015 2.93333 0.133
39 4 0.101 0.024 4.20833 0.230
40 4 0.085 0.033 2.57576 0.227
*
1=Wallasey, 2=Pier Head, 3=Tranmere, 4=Control
2. Study 4, digestive gland data
ROW Label* CDNB PNPA EA
1 4 0.33450 0.09025 -0.0009
2 50 0.23250 0.05925 0.0066
3 63 0.65550 0.12175 0.0056
4 90 0.25300 0.00225 -0.0029
5 69 0.66000 0.17975 0.0396
6 132 0.45850 0.06080 -0.0009
7 17 1.08850 0.17780 0.0121
8 156 1.01200 0.16280 0.0006
9 123 1.09450 0.14530 0.0071
10 103 0.07945 0.13530 0.0026
11 17 0.14050 0.06575 -0.0059
12 29 0.04950 0.07775 -0.0074
13 46 0.23000 0.05525 0.0016
14 3 0.09600 0.13525 0.0081
15 62 0.11250 0.13175 -0.0009
16 45 0.48050 0.10930 0.0068
17 90 0.56080 0.05880 0.0063
18 31 0.44800 0.09180 0.0098
19 42 0.79150 0.05130 0.0083
20 7 0.34450 0.06380 0.0068
21 32 0.65600 0.03725 0.0101
22 44 0.26950 0.06125 0.0001
23 69 0.18850 0.08025 -0.0094
24 22 0.33100 0.08375 0.0206
25 67 0.31150 0.12225 0.0131
26 95 0.52400 0.12030 0.0093
27 53 0.38950 0.11680 0.0241
28 82 0.74950 0.05730 0.0048
29 56 0.21600 0.11580 0.0058
30 15 0.33300 0.07430 0.0088
*
The first 10 figures relate to control values, the second 10 to values from downstream site, the third 10 to upstream site.
3. Study 4, Gill data.
ROW Sample* CDNB PNPA EA
1 123 0.48125 0.061111 0.0159149
2 90 0.42483 0.056771 0.0167830
3 108 0.25816 0.029861 *
4 132 0.27812 0.025521 *
5 156 0.38924 0.029861 *
6 63 0.24913 0.043750 -0.0090278
7 4 0.35764 0.058507 0.0248264
8 69 0.20139 0.024653 0.0118056
9 97 0.34722 0.046354 0.0230903
10 50 0.26215 0.026389 0.0361111
11 98 0.16528 0.015972 -0.0092593
12 31 0.52205 -0.008333 0.0089705
13 90 0.29375 -0.001389 *
14 42 0.24601 -0.000521 *
15 84 0.46649 -0.002257 *
16 34 0.14497 0.032465 0.0100694
17 75 0.18490 0.171875 -0.0185764
18 41 0.05990 0.003819 -0.0159722
19 46 0.22049 0.021181 0.0230903
20 87 0.12587 0.037674 0.0352431
21 48 0.49948 0.025521 0.0124427
22 95 0.52378 0.203125 -0.0196753
23 39 0.69392 0.015972 *
24 77 0.24427 0.016493 *
25 67 0.34288 0.028125 *
26 23 0.25521 0.022049 0.0100694
27 79 0.51562 0.094618 0.0343750
28 69 0.39062 0.025521 -0.0081597
29 49 0.31597 0.026389 0.0309028
30 61 1.17708 0.060243 0.0222222
*
The first 10 figures relate to control values, the second 10 to values from downstream site, the third 10 to upstream site.
4. Study 6 results.
ROW D 0 GST D 0 GSH D 1 mg/l D 1 GST D 1 GSH
1 34.5281 551.68 0 17.5946 559.12
2 34.8270 219.36 0 16.5651 1000.16
3 18.4670 1083.96 0 41.1930 594.80
4 37.8888 514.57 0 30.9657 891.33
5 25.7172 687.49 0 21.3975 1057.44
6 28.5816 1008.45 0 39.1736 86.69
7 25.2298 849.45 0 28.1055 440.94
8 25.7366 990.31 0 35.4470 715.04
9 23.6879 757.24 0 35.4673 675.62
10 35.3682 423.20 0 42.0473 635.80
11 29.1566 223.58 4 20.6982 1089.09
12 28.4110 471.22 4 17.2728 1258.95
13 4 26.6802 386.47
14 4 31.0964 578.04
15 4 36.2194 601.01
16 4 40.2820 655.33
17 4 25.7059 839.49
18 4 41.4128 1208.18
19 4 22.8185 537.98
20 4 25.7402 591.38
21 8 22.4363 828.79
22 8 34.4095 730.66
23 8 35.0046 1383.12
24 8 34.7426 875.63
25 8 29.3586 1650.79
26 8 26.5059 915.76
27 8 28.4781 642.94
28 8 35.1187 804.77
29 8 20.8746 547.65
30 8 31.5169 1203.53
31 12 22.7559 426.01
32 12 23.4799 791.80
33 12 45.4237 346.67
34 12 44.0128 777.56
35 12 35.5802 566.85
36 12 43.1736 588.94
37 12 25.6043 608.47
38 12 29.2432 545.97
39 12 30.2528 267.57
40 12 28.5333 703.03
ROW 4+7 mg/l D 4 GST D 4 GSH D7 GST D 7 GSH
1 0 29.9183 1333.76 28.5933 749.45
2 0 36.0261 656.75 22.3174 738.38
3 0 34.1885 832.98 31.7081 1777.88
4 0 31.6195 1185.07 32.6311 1159.09
5 0 15.4586 431.18 15.0996 504.54
6 0 38.8033 450.96 36.8022 477.58
7 0 34.7170 425.97 33.0933 342.06
8 0 28.8511 476.39 29.9028 262.21
9 0 29.4988 655.47 38.2234 300.78
10 0 18.6674 416.12 25.0400 433.14
11 0 31.0403 545.55 16.3201 592.29
12 0 25.9945 582.66 34.4916 326.35
13 4 20.5205 877.71 46.9096 689.60
14 4 22.9177 457.06 36.9184 672.34
15 4 27.5712 341.88 30.6412 430.11
16 4 39.6072 265.89 15.6008 739.81
17 4 22.0488 659.97 43.3115 655.88
18 4 43.2587 445.69 44.0332 593.38
19 4 31.8280 312.74 26.3511 1038.01
20 4 35.9486 902.63 34.4024 447.94
21 4 23.5021 676.87 27.5800 360.84
22 4 34.3992 805.32 54.8880 1017.84
23 4 42.6979 902.46 32.9755 423.67
24 4 31.6965 1009.57 29.0547 614.46
25 8 41.6537 529.43 33.3495 463.15
26 8 31.9556 784.37 28.0868 736.92
27 8 31.4918 1035.89 40.4225 1057.29
28 8 38.3088 1147.19 51.9410 645.18
29 8 39.8800 604.00 52.5536 665.77
30 8 38.8818 428.22 50.6763 664.54
31 8 42.4213 607.18 34.9762 559.00
32 8 29.7679 750.44 48.9237 639.79
33 8 28.2227 447.17 31.6947 720.83
34 8 35.5275 883.21 50.6319 371.87
35 8 25.6669 621.85 29.0836 561.21
36 8 27.5475 653.58 28.0252 580.25
37 12 34.5681 1022.72 23.4602 548.45
38 12 19.2282 467.40 37.1821 563.16
39 12 28.3043 873.55 47.7359 427.65
40 12 35.8211 506.63 38.2817 1266.93
41 12 25.1073 428.89 36.6916 819.91
42 12 15.9614 217.23 28.5159 1377.26
43 12 21.4543 1228.48 29.0585 279.03
44 12 39.4452 532.95 42.9049 905.98
45 12 13.2422 385.08 50.6832 661.18
46 12 38.3248 705.27 32.2971 843.23
47 12 27.6394 405.70 26.8320 556.28
48 12 37.8258 659.11 33.5007 598.25